Contact an Account ManagerFully automated online solid phase extraction coupled directly to liquid chromatography-tandem mass spectrometry Quantification of sulfonamide antibiotics
Summary
Type: HPLC
Application #: 121350
Catalog#: 760100.20 - MN HPLC Column, Nucleodur C18 Gravity, 110A, 5um, 2x125mm
Detection: LC-MS/MS (ESI positive mode, spray voltage 3500 V, sheath gas 40 bar, auxiliary gas 5 bar, ion transfer capillary temperature 350 °C)
Conditions: Online SPE: Please see literature
HPLC:
Eluent: A: Water, 20 mM formic acid, pH 2.7;
B: methanol;
C: water, 10 mM ammonia acetate, pH 7
Gradient and flow rate:
0. min 5%A 5%B 90%C 400 μl/min
4. min 5%A 5%B 90%C 400 μl/min
4.1. min 20%A 20%B 60%C 250 μl/min
20. min 40%A 40%B 20%C 250 μl/min
22. min 0%A 80%B 20%C 250 μl/min
26. min 20%A 20%B 60%C 250 μl/min
28. min 5%A 5%B 90%C 400 μl/min
33. min 5%A 5%B 90%C 400 μl/min
Authors: Stoob K et al
Source: Swiss Federal Institute of Aquatic Science and Tec